Multiple Choice Questions 10×1=10
1
What can happen if an enzyme is exposed to temperature that is higher than its optimal temperature?
A
Enzyme activity rate will increase
B
Enzyme’s shape will change, potentially reducing its activity
C
Enzyme will speed up the reaction and remain stable
D
Enzyme will become a substrate itself
2
What enzymes are primarily composed of:
A
Carbohydrates
B
Lipids
C
Proteins
D
Nucleic acid
3
Where do intracellular enzymes function?
A
Outside the cell
B
Within the cells
C
In the bloodstream
D
In the digestive system
4
Which enzyme is used in biological detergents to remove protein stains?
A
Amylase
B
Protease
C
Lipase
D
Lactase
5
……… tightly bind with enzymes:
A
Coenzymes
B
Prosthetic groups
C
Cofactors
D
Ribozyme
6
What is the term used to describe the loss of an enzyme’s globular structure due to high temperature?
A
Ionization
B
Denaturation
C
Saturation
D
None of these
7
What is the optimum pH for trypsin?
A
1.5 to 2.0
B
5.0 to 6.0
C
7.0 to 8.0
D
9.0 to 10.0
8
What is the primary factor that affect the ionization of amino acids at the active site of an enzyme?
A
Temperature
B
Substrate concentration
C
pH
D
Enzyme concentration
9
Which type of inhibitor resembles the enzyme’s substrate?
A
Non-competitive inhibitor
B
Competitive inhibitor
C
Allosteric inhibitor
D
Feedback inhibitor
10
Which of the following is an example of a competitive inhibitor?
A
Heavy metals
B
Antibiotics
C
Cancer drugs
D
Vitamins
Short Answer Questions 15×2=30
I
Define an enzyme. What is its role in metabolism?
II
What is the effect of substrate concentration on enzyme activity?
III
Which type of enzyme inhibitors inhibit the enzymes without attaching to the active site?
IV
Differentiate between competitive and non-competitive inhibition.
VII
What is enzyme specificity?
Hint: Enzymes are highly specific for their substrates
VIII
What are cofactors in enzymes?
IX
What is the difference between prosthetic groups and coenzymes?
X
What is the active site of an enzyme?
XI
Who proposed the induced fit model?
XII
What is the role of the substrate in enzyme action?
XIII
What is optimum temperature?
XIV
What is denaturation of enzyme?
XV
Where does a non-competitive inhibitor bind to the enzyme?